• Product Namep44/42 MAP Kinase(Ab-204) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total p44/42 MAP Kinase protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 202~206 (T-E-Y-V-A) derived from Human p44/42 MAP Kinase.
• Target Namep44/42 MAP Kinase
• Alternative Names    Extracellular signal-regulated kinase 1
• Accession No. Swiss-Prot: P27361
  NCBI Protein: NP_001035145.1
• UniprotP27361
• Gene ID5595;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 42 44 kd
  Western blotting: 1:500~1:1000
   

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1

TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397
Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162
Jingui Yu, et al. (2005) Anesth Analg 101: 315-321
Hironobu Ihn et al.(2000) Immunology 165: 2149-2155
 

• Western blot analysis of extracts from PC12 and 293 cells using p44/42 MAP Kinase(Ab-204) Antibody #21238.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting