• Product NameARAF antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationPurified by antigen-affinity chromatography.
• ApplicationsWB IHC IF
• Species ReactivityHu
• Immunogen TypeRecombinant protein
• Immunogen DescriptionRecombinant protein fragment contain a sequence corresponding to a region within amino acids 371 and 524 of ARAF
• Target NameARAF
• Accession No. Swiss-Prot:P10398
  Gene ID:369
• UniprotP10398
• Gene ID369;
• Concentration 0.9mg/ml
• Formulation Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Storage Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

• Predicted MW: 68kd
  Western blotting: 1:500-1:3000
  Immunohistochemistry: 1:50-1:500
  Immunofluorescence: 1:100-1:200
  

This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. [provided by RefSeq]

• Sample (30 ug of whole cell lysate)
  A: Hela
  7.5% SDS PAGE
  Primary antibody diluted at 1: 1000
• Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF antibody at 1: 500 dilution.
• Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF antibody at 1: 200 dilution.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting