• Product NamePTPN14 Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Clone No.H7-B3
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, FC
• Species ReactivityHu
• Immunogen DescriptionRecombinant protein
• Alternative Names    Cytoskeletal associated protein tyrosine phosphatase antibody
  MGC126803 antibody
  PEZ antibody
  Phosphatase with ezrin domain antibody
  Protein tyrosine phosphatase non receptor type 14 antibody
  Protein tyrosine phosphatase pez antibody
  Protein-tyrosine phosphatase pez antibody
  PTN14_HUMAN antibody
  PTP 36 antibody
  PTP36 antibody
  PTPD 2 antibody
  PTPN 14 antibody
  PTPN14 antibody
  Tyrosine protein phosphatase non receptor type 14 antibody
  Tyrosine-protein phosphatase non-receptor type 14 antibody
  
• Accession No. Swiss-Prot#:Q15678
• UniprotQ15678
• Gene ID5784;
• Calculated MW 135 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:500-1:2,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  FC: 1:50-1:100

Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.

• Western blot analysis of PTPN14 on human PTPN14 ecombinant protein using anti-PTPN14 antibody at 1/1,000 dilution.
• Western blot analysis of PTPN14 on HEK293 (1) and PTPN14-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPN14 antibody at 1/1,000 dilution.
• Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PTPN14 antibody. Counter stained with hematoxylin.
• ICC staining PTPN14 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with PTPN14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting