• Product NamePhospho-eIF4E (S209) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.SU0396
• PurificationProA affinity purified
• ApplicationsWB, ICC, IHC, IP
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionSynthetic phospho-peptide corresponding to residues surrounding Ser209 of human eIF4E.
• Alternative Names    AUTS19 antibody
  CBP antibody
  eIF 4E antibody
  eIF 4F 25 kDa subunit antibody
  EIF 4F antibody
  eIF-4E antibody
  eIF-4F 25 kDa subunit antibody
  eIF4E antibody
  EIF4E1 antibody
  EIF4EL1 antibody
  EIF4F antibody
  Eukaryotic translation initiation factor 4 E antibody
  Eukaryotic translation initiation factor 4E antibody
  Eukaryotic translation initiation factor 4E like 1 antibody
  IF4E_HUMAN antibody
  Messanger RNA Cap Binding Protein eIF 4E antibody
  MGC111573 antibody
  mRNA cap binding protein antibody
  mRNA cap-binding protein antibody
  
• Accession No. Swiss-Prot#:P06730
• UniprotP06730
• Gene ID1977;
• Calculated MW 25 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-5,000
  IHC: 1:50-1:200
  ICC: 1:50-1:200
  
  

The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4E, eIF4A and eIF4G. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E binds to the mRNA "cap" during an early step in the initiation of protein synthesis. eIF4A acts as an ATP-dependent RNA helicase. eIF4G acts as a bridge between eIF4E, eIF4A and the eIF3 complex.

• Western blot analysis of Phospho-eIF4E(S209) on different lysates using anti-Phospho-eIF4E(S209) antibody at 1/1,000 dilution. Positive control:
  Lane 1: Mouse spleen
  Lane 2: Rat spleen
• Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
• Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E(S209) antibody. Counter stained with hematoxylin.
• ICC staining Phospho-eIF4E(S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho-eIF4E(S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Phospho-eIF4E(S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting