• Product NameAurora A Rabbit mAb
• Host SpeciesRecombinant Rabbit
• Clonality Monoclonal antibody
• Clone No.ST46-07
• PurificationProA affinity purified
• ApplicationsWB, ICC/IF, FC
• Species ReactivityHu, Ms
• Immunogen Descriptionrecombinant protein
• Alternative Names    AIK antibody
  ARK-1 antibody
  ARK1 antibody
  AURA antibody
  Aurka antibody
  Aurora 2 antibody
  Aurora A antibody
  Aurora kinase A antibody
  Aurora-related kinase 1 antibody
  Aurora/IPL1 like kinase antibody
  Aurora/IPL1-related kinase 1 antibody
  AURORA2 antibody
  Breast tumor-amplified kinase antibody
  BTAK antibody
  hARK1 antibody
  IAK antibody
  IPL1 related kinase antibody
  MGC34538 antibody
  OTTHUMP00000031340 antibody
  OTTHUMP00000031341 antibody
  OTTHUMP00000031342 antibody
  OTTHUMP00000031343 antibody
  OTTHUMP00000031344 antibody
  OTTHUMP00000031345 antibody
  OTTHUMP00000166071 antibody
  OTTHUMP00000166072 antibody
  PPP1R47 antibody
  Protein phosphatase 1, regulatory subunit 47 antibody
  Serine/threonine kinase 15 antibody
  Serine/threonine kinase 6 antibody
  Serine/threonine-protein kinase 15 antibody
  Serine/threonine-protein kinase 6 antibody
  Serine/threonine-protein kinase aurora-A antibody
  STK15 antibody
  STK6 antibody
  STK6_HUMAN antibody
  STK7 antibody
  
• Accession No. Swiss-Prot#:O14965
• UniprotO14965
• Gene ID6790;
• Calculated MW 46 kDa
• Formulation 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• Storage Store at -20˚C

• WB: 1:1,000-1:2,000
  
  ICC: 1:100-1:500
  
  FC: 1:50-1:100

Activation of the oncogenic protein kinase Aurora A regulates meiotic and mitotic cell cycles in eukaryotic cells. Specifically, Aurora A plays a key role in G2/M progression. Activation occurs via autophosphorylation, and while 14 sites are subject to this, only the threonine residue at position 295 is required for activity. Though autophosphorylation mediates activation, a number of other proteins influence activation, including the spindle assembly factor TPX2 and p53

• ICC staining Aurora A in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Aurora A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• ICC staining Aurora A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
• Flow cytometric analysis of Hela cells with Aurora A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting