Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate WNT9B in samples. An antibody specific for WNT9B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT9B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT9B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT9B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:WNT9B is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.WNT15 shows 53% amino acid identity to chicken Wnt14, 54% identity to human WNT14, and 65% identity to shark Wnt9. The deduced 359-amino acid protein shares significant identity with the 331-amino acid human WNT9B protein, and both proteins contain all 23 cysteines conserved in Wnt proteins. Northern blot analysis of adult mouse tissues detected highest expression in kidney and lower expression in liver, brain, male preputial gland, and female mammary gland. No expression was detected in other adult tissues examined.
