Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TXNIP in samples. An antibody specific for TXNIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTXNIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TXNIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TXNIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Exposure to vitamin D3 (1,25-dihydroxyvitamin D3) or phorbol ester induces the bipotent HL-60 cell promyelocytic leukemia cell line to differentiate into monocytes/macrophages, whereas retinoic acid and dimethylsulfoxide induce differentiation towards granulocytes. The differentiation is accompanied by regulation of MYC , FOS , FMS (CSF1R), and myeloblastin (PRTN3;). By differential screening of HL60 cell lines, Chen and DeLuca (1994) identified a cDNA encoding TXNIP, which they termed VDUP1. The deduced TXNIP protein has 391 amino acids. Ribonuclease protection analysis showed dramatically increased expression of TXNIP in response to vitamin D3 but not to phorbol ester. Chen and DeLuca (1994) concluded that TXNIP is not involved in the differentiation process.
