Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TNFAIP2 in samples. An antibody specific for TNFAIP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The TNFAIP2 gene was induced in HUVECs in response to the proinflammatory molecules TNF, IL1B , and lipopolysaccharide. TNFAIP2 demonstrated the characteristics of a primary response gene in its rapid and substantial induction in the absence of intermediary protein synthesis. The authors found that TNFAIP2 transcription was induced during capillary tube formation in vitro. Northern blot analysis detected a 4.2-kb human TNFAIP2 transcript. During mouse development, expression of the Tnfaip2 transcript varied in a temporal fashion that was organ-specific. The TNFAIP2 cDNA encodes a predicted 654-amino acid protein with a single N-linked glycosylation site; stretches of glutamates, lysines, and alanines near the N terminus; and 4 glutamines near the C terminus.
