• Product NameFructose-bisphosphate aldolase A Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Fructose-bisphosphate aldolase A polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Fructose-bisphosphate aldolase A protein
• Target NameFructose-bisphosphate aldolase A
• Alternative Names    ALDOA, ALDA, Lung cancer antigen NY-LU-1, Muscle-type aldolase
• Accession No. Swiss-Prot#: P04075
• UniprotP04075
• Gene ID226;
• Calculated MW 40kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein .Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.Belongs to the class I fructose-bisphosphate aldolase family.

[1]"Nucleotide sequence of a cDNA clone for human aldolase: a messenger RNA in the liver." Sakakibara M., Mukai T., Hori K. Biochem. Biophys. Res. Commun. 131:413-420(1985) [2]"A new human species of aldolase A mRNA from fibroblasts." Izzo P., Costanz

• Fructose-bisphosphate aldolase A polyclonal Antibody at 2ug/ml + EC109 whole cell lysate at 20ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 40 kDa Observed band size : 40 kDa
• Immunohistochemical analysis of paraffin-embeded human lung cancer using #42051 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting