• Product NamePhosphoglycerate kinase 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total Phosphoglycerate kinase 1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Phosphoglycerate kinase 1 protein
• Target NamePhosphoglycerate kinase 1
• Alternative Names    PGK1
• Accession No. Swiss-Prot#: P00558
• UniprotP00558
• Gene ID5230;
• Calculated MW 46kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. In addition to its role as a glycolytic enzyme, PGK-1 appears to acts as a polymerase alpha cofactor protein. Defects in PGK1 are generally associated with chronic hemolytic anemia.

[1] "Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase."Michelson A.M., Markham A.F., Orkin S.H.Proc. Natl. Acad. Sci. U.S.A. 80:472-476(1983) [2] "Structure of the human phosphoglycerate kina

• All lanes : Phosphoglycerate kinase 1 antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysateSecondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 46kDa Observed band size: 46kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting