• Product NameV-type proton ATPase subunit G 1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total V-type proton ATPase subunit G 1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human V-type proton ATPase subunit G 1 protein
• Target NameV-type proton ATPase subunit G 1
• Alternative Names    V-ATPase 13 kDa subunit 1; Vacuolar proton pump subunit G 1; Vacuolar proton pump subunit M16
• Accession No. Swiss-Prot#: O75348
• UniprotO75348
• Gene ID9550;
• Calculated MW 13kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

This protein is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.

[1]"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu

• All lanes:V-type proton ATPase subunit G 1 antibody at 2ug/ml+ 293T whole cell lysate at 20ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 13 kDa Observed band size: 13kDa
• Immunohistochemical analysis of paraffin-embeded colorectal carcinoma using #42060 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting