• Product NameCytochrome c oxidase subunit 5A, mitochondrial Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Cytochrome c oxidase subunit 5A, mitochondrial polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Cytochrome c oxidase subunit 5A, mitochondrial protein
• Target NameCytochrome c oxidase subunit 5A, mitochondrial
• Alternative Names    COX5A, Cytochrome c oxidase polypeptide Va
• Accession No. Swiss-Prot#: P20674
• UniprotP20674
• Gene ID9377;
• Calculated MW 16.5kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

[1] "Subunit Va of human and bovine cytochrome c oxidase is highly conserved."Rizzuto R., Nakase H., Zeviani M., Dimauro S., Schon E.A.Gene 69:245-256(1988) [2] "Molecular evolution of the cytochrome c oxidase subunit 5A gene in primates."Uddin M., Opaz

• All lanes : Cytochrome c oxidase subunit 5A, mitochondrial antibody at 2ug/ml+293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 16.5 kDa Observed band size: 16.5kDa
• Immunohistochemical analysis of paraffin-embedded human brain using #42096 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting