• Product NameCHRNA1 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityTorpedo californica
• SpecificityThe antibody detects endogenous level of total CHRNA1 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Torpedo californica Acetylcholine receptor subunit alpha protein
• Target NameCHRNA1
• Alternative Names    CHRNA1
• Accession No. Swiss-Prot#: P02710
• UniprotP02710
• Calculated MW 53kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

[1]"Compared structures of the free nicotinic acetylcholine receptor main immunogenic region (MIR) decapeptide and the antibody-bound [A76]MIR analogue: a molecular dynamics simulation from two-dimensional NMR data."Orlewski P., Marraud M., Cu

• All lanes:Acetylcholine receptor subunit alpha antibody at 2ug/ml+recombinant Acetylcholine receptor subunit alpha protein 0.1ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size:53KDa Observed band size:53KDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting