• Product NameADP-ribosylation factor 4 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total ADP-ribosylation factor 4 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human ADP-ribosylation factor 4 protein
• Target NameADP-ribosylation factor 4
• Alternative Names    ARF4, ARF2
• Accession No. Swiss-Prot#: P18085
• UniprotP18085
• Gene ID378;
• Calculated MW 20kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

[1]"Selective amplification of an mRNA and related pseudogene for a human ADP-ribosylation factor, a guanine nucleotide-dependent protein activator of cholera toxin."Monaco L., Murtagh J.J. Jr., Newman K.B., Tsai S.-C., Moss J., Vaughan M.Proc. Natl. Acad

• All lanes : ADP-ribosylation factor 4 antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 20 kDa Observed band size: 20 kDa
• Immunohistochemical analysis of paraffin-embeded human colorectal carcinoma using #42173 at dilution of 1:50.
• Immunohistochemical analysis of paraffin-embeded human kidney using #42173 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting