• Product NameGDP-L-fucose synthase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total GDP-L-fucose synthase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human GDP-L-fucose synthase protein
• Target NameGDP-L-fucose synthase
• Alternative Names    GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase Protein FX Red cell NADP(H)-binding protein Short-chain dehydrogenase/reductase family 4E member 1
• Accession No. Swiss-Prot#: Q13630
• UniprotQ13630
• Gene ID7264;
• Calculated MW 35kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.

[1] "Synthesis of GDP-L-fucose by the human FX protein."Tonetti M., Sturla L., Bisso A., Benatti U., De Flora A.J. Biol. Chem. 271:27274-27279(1996) [2] "Complete sequencing and characterization of 21,243 full-length human cDNAs."Ota T., Suzuki Y., Nis

• Western blot ALL Lanes:GDP-L-fucose synthase Antibody at 2 ug/ml + EC109 whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 35 kDa Observed band size : 35 kDa Additional bands at : 27 kDa. We are unsure as to the identity of this extra band.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting