• Product NameGastric intrinsic factor Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Gastric intrinsic factor polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Gastric intrinsic factor protein£¨19-417aa£©
• Target NameGastric intrinsic factor
• Alternative Names    Intrinsic factor, IF, INF, IFMH, GIF
• Accession No. Swiss-Prot#: P27352
• UniprotP27352
• Gene ID2694;
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:20 - 1:200

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis.

[1]"Hereditary juvenile cobalamin deficiency caused by mutations in the intrinsic factor gene." Tanner S.M., Li Z., Perko J.D., Oener C., Cetin M., Altay C., Yurtsever Z., David K.L., Faivre L., Ismail E.A., Graesbeck R., de la Chapelle A. Proc. Natl. Acad. Sci. U.S.A. 102:4130-4133(2005). [2]"A genetic polymorphism in the coding region of the gastric intrinsic factor gene (GIF) is associated with congenital intrinsic factor deficiency." Gordon M.M., Brada N., Remacha A., Badell I., del Rio E., Baiget M., Santer R., Quadros E.V., Rothenberg S.P., Alpers D.H. Hum. Mutat. 23:85-91(2004). [3]"Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes." Andersen C.B., Madsen M., Storm T., Moestrup S.K., Andersen G.R. Nature 464:445-448(2010).

• Immunohistochemical analysis of paraffin-embedded human lung tissue using #42180 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting