• Product NameBub3 Conjugated Antibody
• Host SpeciesRabbit
• ClonalityMonoclonal
• Species ReactivityHu, Ms, Rt
• Immunogen DescriptionRecombinant protein corresponding to the N-terminus of human Bub3.
• ConjugatesBiotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation EmissionAF350: 346nm/442nm
  AF405: 401nm/421nm
  AF488: 493nm/519nm
  AF555: 555nm/565nm
  AF594: 591nm/614nm
  AF647: 651nm/667nm
  AF680: 679nm/702nm
  AF750: 749nm/775nm
• Alternative Names    Aa2-050 antibody
  AU019800 antibody
  AU021329 antibody
  AU043350 antibody
  AW146323 antibody
  BUB 3 antibody
  BUB 3L antibody
  BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
  bub3 antibody
  BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
  BUB3 mitotic checkpoint protein antibody
  BUB3_HUMAN antibody
  BUB3L antibody
  Budding uninhibited by benomyl antibody
  Budding uninhibited by benzimidazoles 3 antibody
  Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
  Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
  Budding uninhibited by benzimidazoles 3 homolog antibody
  Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
  C78067 antibody
  hBUB3 antibody
  Mitotic checkpoint component antibody
  Mitotic checkpoint protein bub3 antibody
  OTTHUMP00000020679 antibody
  OTTHUMP00000020680 antibody
  WD repeat type I transmembrane protein A72.5 antibody
  
• Accession No. Swiss-Prot#:O43684
  
• UniprotO43684
• Gene ID9184;
• Calculated MW 37 kDa
• Formulation 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
• Storage Store at 4˚C in dark for 6 months

• Suggested Dilution:
  AF350 conjugated: most applications: 1: 50 - 1: 250
  AF405 conjugated: most applications: 1: 50 - 1: 250
  AF488 conjugated: most applications: 1: 50 - 1: 250
  AF555 conjugated: most applications: 1: 50 - 1: 250
  AF594 conjugated: most applications: 1: 50 - 1: 250
  AF647 conjugated: most applications: 1: 50 - 1: 250
  AF680 conjugated: most applications: 1: 50 - 1: 250
  AF750 conjugated: most applications: 1: 50 - 1: 250
  Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting