• Product NameMEK1/MEK2(Phospho-Ser217/Ser221) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIHC WB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of MEK1/MEK2 only when phosphorylated at serine 217/221.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 217/221 (I-D-S(p)-M-A) derived from Human MEK1/MEK2.
• Target NameMEK1/MEK2
• ModificationPhospho
• Alternative Names    ERK activator kinase 1/ERK activator kinase2; MAP kinase kinase 1/MAP kinase kinase 2; MAP2K1/MAP2K1; MAPK/ERK kinase 1/MAPK/ERK kinase 2; MAPKK 1/MAPKK 1
• Accession No. Swiss-Prot: Q02750
  NCBI Protein: NP _002746.1 NP _109587.1
• UniprotQ02750
• Gene ID5604;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 45kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Shen R, et al. (2002) Mol Cell Biol; 22(10): 3230-3236
Preisinger C, et al. (2005) EMBO J; 24(4): 753-765
Laine P, et al. (2000) Biochem J; 349(Pt 1): 19-25
Yaglom J, et al. (2003) Mol Cell Biol; 23(11): 3813-3824
Dahan S, et al. (2002) Infect Immun; 70(5): 2304-2310

• Western blot analysis of extracts from Hela cell untreated or treated with UV MEK1/MEK2(Phospho-Ser217/Ser221) Antibody#11205
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2(Phospho-Ser217/Ser221) Antibody #11205(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting