• Product NamePAKβ (phospho Ser154) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF(paraffin section),ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-PAKβ (S154) Polyclonal Antibody detects endogenous levels of PAKβ protein only when phosphorylated at S154.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human PAK3 around the phosphorylation site of Ser154. AA range:121-170
• Alternative Names    PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3
• Accession No. Swiss Prot:O75914
  GeneID:5063
• UniprotO75914
• Gene ID5063
• SDS-PAGE MW 72
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  ELISA: 1/20000. Not yet tested in other applications.

p21 (RAC1) activated kinase 3(PAK3) Homo sapiens The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Apr 2016],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting