• Product NameIκB-ε (phospho Ser22) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. AA range:131-180
• Alternative Names    NFKBIE; IKBE; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; I-kappa-B-epsilon; IkB-E; IkB-epsilon; IkappaBepsilon
• Accession No. Swiss Prot:O00221
  GeneID:4794
• UniprotO00221
• Gene ID4794
• SDS-PAGE MW 38
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/40000. Not yet tested in other applications.

NFKB inhibitor epsilon(NFKBIE) Homo sapiens The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting