• Product NameCaMKIα (phospho Thr177) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,IHC-p,IF/ICC,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192
• Alternative Names    CAMK1; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha
• Accession No. Swiss Prot:Q14012
  GeneID:8536
• UniprotQ14012
• Gene ID8536
• SDS-PAGE MW 41
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  Immunohistochemistry: 1/100 - 1/300.
  Immunofluorescence: 1/200 - 1/1000.
  ELISA: 1/5000. Not yet tested in other applications.

calcium/calmodulin dependent protein kinase I(CAMK1) Homo sapiens Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting