• Product NameC/EBP α (phospho Thr226) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,ELISA
• Species ReactivityHuman,Mouse,Rat,Monkey
• SpecificityPhospho-C/EBP α (T226) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at T226.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Thr226. AA range:192-241
• Alternative Names    CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
• Accession No. Swiss Prot:P49715
  GeneID:1050
• UniprotP49715
• Gene ID1050
• SDS-PAGE MW 37
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000. Not yet tested in other applications.

CCAAT/enhancer binding protein alpha(CEBPA) Homo sapiens This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting