• Product Name14-3-3 β/ζ (phospho Ser184/186) Polyclonal Antibody
• Host SpeciesRabbit
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB,ELISA
• Species ReactivityHuman,Mouse,Rat
• SpecificityPhospho-14-3-3 β/ζ (S184/186) Polyclonal Antibody detects endogenous levels of 14-3-3 β/ζ protein only when phosphorylated at S184/186.
• Immunogen DescriptionThe antiserum was produced against synthesized peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of Ser184/186. AA range:151-200
• Alternative Names    YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1; YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1
• Accession No. Swiss Prot:P63104/P31946
  GeneID:7534/7529
• UniprotP63104/P31946
• Gene ID7534/7529
• SDS-PAGE MW 28
• Concentration 1 mg/ml
• Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Storage -20˚C/1

• Western Blot: 1/500 - 1/2000.
  ELISA: 1/5000. Not yet tested in other applications.

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta(YWHAZ) Homo sapiens This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting