• Product NameNucleolin Rabbit mAb
• Host SpeciesRecombinant Rabbit
• ClonalityMonoclonal antibody
• Clone No.S08-6H3
• IsotypeIgG
• PurificationAffinity Purified
• ApplicationsWB
• Species ReactivityHuman,Mouse,Rat
• Immunogen DescriptionA synthetic peptide of human Nucleolin
• ConjugatesUnconjugated
• ModificationUnmodification
• Alternative Names    C23; Nsr1
• Accession No. Swiss-Prot:P19338
  GeneID:4691
• UniprotP19338
• Gene ID4691
• Calculated MW Calculated MW:77 kDa,Observed MW:100 kDa
• Concentration 0.3 mg/ml
• Formulation 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Storage Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.

• WB: 1/2000-1/10000

Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

• Western blot detection of Nucleolin in Hela,A549,HL-60 using Nucleolin Rabbit mAb(1:1000 diluted)

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting