- Product NameRecombinant Human/Murine/Rat/Canine/Equine Brain-derived Neurotrophic Factor
- Host SpeciesSpodoptera frugiperda, Sf21 (baculovirus).
- Purification> 97 % by SDS-PAGE analyses.
- Biological ActivityMeasured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL. The specific activity of rHu/Mu/Rt/Ca/EqBDNF is approximately 1.3 x 103units/ug, which is calibrated against recombinant human BDNF WHO Standard. Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera is coated at 1 ug/mL, rHu/Mu/Rt/Ca/EqBDNF binds with an apparent Kd <1 nM.
- EndotoxinLess than 0.1 EU/ug of rHu/Mu/Rt/Ca/EqBDNF as determined by LAL method.
- Calculated MW Apparent molecular mass of 13-14 kDa in SDS-PAGE under reducing conditions, a single glycosylated polypeptide protein consisting of 119 amino acids.
- Target Sequence His129-Arg247; Accession # P23560
- Formulation Lyophilized from 0.2 μm filtered concentrated solution in 100 mM Sodium Citrate and 300 mM NaCl, pH 3.0.
- Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 ˚C as supplied.- 1 month, 2 to 8 ˚C under sterile conditions after reconstitution.- 3 months, -20 to -70 ˚C under sterile conditions after reconstitution.
