• Product NameNF-κB (p105/p50) Rabbit mAb
• Host SpeciesRabbit
• ClonalityMonoclonal
• Clone No.AB9
• IsotypeRabbit IgG
• PurificationAffinity-chromatography
• ApplicationsWB IHC
• Species ReactivityHuman Mouse Rat
• SpecificityNF-κB (p105/p50) Antibody detects endogenous levels of total NF-κB (p105/p50)
• Immunogen DescriptionA synthesized peptide derived from human NF-κB (p105/p50)
• Target NameNFKB1
• Alternative Names    NF-kappa-B1; p84/NF-kappa-B1 p98; NFKB1; DNA-binding factor KBF1; EBP-1; NFkB-p50;
• Accession No. Uniprot:P19838
• UniprotP19838
• Calculated MW 50,120 kDa
• Formulation Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at +4˚C in dark for 6 months.

• WB 1:500~1:2000 IHC 1:50~1:200

• NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

• All Lanes: NF-κB p105/p50 Antibody at 1/ 1000 dilution.Lane1:PC-12 cell lysate.Lane2:NIH/3T3 cell lysate.(Proteins at 20ug per lane)Secondary: Goat Anti-Rabbit IgG(HRP) at 1/20000 dilutionPredicted band size : 50kDa/120kDaObserved band size: 50kDa/120kDa
• Immunohistochemical analysis of paraffin-embedded human kidney, using NF-κB p105/p50 Antibody.Tris-EDTA Buffer (pH9.0).
• All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting