• Product NameSTAT4(Phospho-Tyr693) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of STAT4 only when phosphorylated at tyrosine 693.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 693 (K-G-Y(p)-V-P) derived from Human STAT4.
• Target NameSTAT4
• ModificationPhospho
• Alternative Names    SLEB11
• Accession No. Swiss-Prot: Q14765
  NCBI Protein: NP_003142.1
• UniprotQ14765
• Gene ID6775;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 85kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

Strausberg R L, et al. (2002) Proc Natl Acad Sci U S A. 99(26):16899-16903.

• Western blot analysis of extracts from HepG2 cells untreated or treated with IL-4 using STAT4(Phospho-Tyr693) Antibody #11047.
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT4(Phospho-Tyr693) Antibody #11047(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting