• Product NameGIP Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen affinity purification.
• ApplicationsWB IHC
• Species ReactivityHu,Ms,Rat
• SpecificityThe antibody detects endogenous levels of total GIP protein.
• Immunogen TypeRecombinant Protein
• Immunogen DescriptionFusion protein corresponding to a region derived from internal residues of human gastric inhibitory polypeptide
• Target NameGIP
• Alternative Names    GIP; Gastric inhibitory polypeptide;Glucose-dependent insulinotropic polypeptide
• Accession No. Swiss-Prot#: P09681
  NCBI Gene ID: 2695
  Gene Accssion: BC069663
• UniprotP09681
• Gene ID2695;
• SDS-PAGE MW 17kd
• Concentration 0.5mg/ml
• Formulation Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• Western blotting: 1:500-1:2000 IHC 1:50 - 1:200

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion.

• Western blot analysis of GIP expression in HepG2 (A), mouse liver (B), rat liver (C) whole cell lysates.
• Immunohistochemical analysis of GIP staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting