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c-Jun(Phospho-Ser73) Antibody#11003

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Product Detail

Product Namec-Jun(Phospho-Ser73) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of c-Jun only when phosphorylated at serine 73.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 73 (L-A-S(p)-P-E) derived from Human c-Jun.

Target Namec-Jun

ConjugateUnconjugated

Other NamesAH119; AP1; Jun A; c-Jun; p39

Accession NoSwiss-Prot: P05412
NCBI Protein: NP_002219.1

Uniprot P05412

Gene ID 3725;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody #11003.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody #11003(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody #11003.

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'.

If you have published an article using product 11003, please notify us so that we can cite your literature.

et al,Increase in claudin-2 expression by an EGFR/MEK/ERK/c-Fos pathway in lung adenocarcinoma A549 cells. In Biochim Biophys Acta on 2012 Jun by Akira Ikari, Tomonari Sato, et al..PMID: 22546605 , (2012),
PMID: 22546605
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987 , (2012),
PMID: 23191987
et al,The pivotal role of SUMO-1-JNK-Tau axis in an in vitro model of oxidative stress counteracted by the protective effect of curcumin. In Biochem Pharmacol on 2020 Aug by Lucia Buccarello, Jessica Dragotto, et al..PMID:32502496 , (2020),
PMID: 32502496

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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