Product NamePDK1(Phospho-Ser241) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 241 (A-N-S(p)-F-V) derived from Human PDK1.

Target NamePDK1

ConjugateUnconjugated

Other NamesPDPK1; PkB kinase; Protein kinase B kinase; hPDK1; kinase PDK1

Accession NoSwiss-Prot: O15530
NCBI Protein: NP_002604.1

Uniprot O15530

Gene ID 5170;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200