Product Detail
Product NameNFκB-p65(Phospho-Ser276) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB;IHC;IF;IP
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of NFκB-p65 only when phosphorylated at serine 276.
Immunogen TypePeptide-KLH
Immunogen Desc: Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NFκB-p65.
Target NameNFκB-p65
ConjugateUnconjugated
Other Namesp65, NFKB3
Accession NoSwiss-Prot#:Q04206 NCBI Gene#:5970 NCBI Protein#:NP_001138610.1
Uniprot
Q04206
Gene ID
5970;
Sdspage MW65KD
Concentration1.0mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
StorageStore at -20 ˚C/1 year
Application Details
WB 1:500 - 1:2000. IHC 1:100 - 1:300 Immunoprecipitation: 2-5 ug:mg lysate IF 1:50-200
Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser276) Antibody #11011.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody #11011 (left) or the same antibody preincubated with blocking peptide #51011 (right).
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody #11011.
Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody #11011.
Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFκB-p65 (Phospho-Ser276) Antibody #11011.
Western Blot analysis of various cells using Phospho-NFκB-p65 (S276) Polyclonal Antibody diluted at 1:1000
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
If you have published an article using product 11011, please notify us so that we can cite your literature.
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et al,Serum Amyloid A Stimulates Vascular and Renal Dysfunction In ApolipoproteIn E-Deficient Mice Fed a Normal Chow Diet. In Front Immunol on 2019 Mar 7 by Chami B, HossaIn F, et al..PMID:30899260
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et al,Apoptosis Induction and AKT/NF-κB Inactivation are associated with regroafenib-Inhibited tumor progression In non-small cell lung cancer In vitro and In vivo. In Biomed Pharmacother on 2019 Jun 1 by Weng MC, Li MH, et al.. PMID:31163381
, (2019),
PMID:
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et al,HyperforIn Induces Apoptosis Through ExtrInsic/IntrInsic Pathways and Inhibits NF-?B-modulated Survival and Invasion Potential In Bladder Cancer. In In Vivo on 2019 Nov-Dec by Liu YC, LIn KH, et al..PMID:31662514
, (2019),
PMID:
31662514
et al,Beneficial effect of fluoxetine on anti-tumor progression on hepatocellular carcinoma and non-small cell lung cancer bearing animal model.In Biomed Pharmacother on 2020 Jun; by Hsu LC, Tu HF, et al..PMID: 32145588
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More
et al,Hunting for Serine 276-Phosphorylated p65. In J Biomed Biotechnol on 2010 by Anneleen Spooren, Krzysztof Kolmus, et al..PMID: 20204068
, (2010),
PMID: 20204068
et al,Serum amyloid A stimulates cultured endothelial cells to migrate and proliferate: inhibition by the multikinase inhibitor BIBF 1120. In Clin Exp Pharmacol Physiol on 2013 Sep by Xiaoping Cai, S Ben Freedman, et al..PMID:23819722
, (2013),
PMID: 23819722
et al,Selenium Inhibits Renal Oxidation and Inflammation But Not Acute Kidney Injury in an Animal Model of Rhabdomyolysis
, (2013),
PMID: 22937747
et al,Sirtuin 7 Promotes Alcohol-Associated Liver Injury via Modulating Myeloid Cell Chemokine (C-C Motif) Ligand 2 Secretion through the NF-kB Signaling Pathway
, (2025),
PMID:
et al,Sirtuin 7 Promotes Alcohol-Associated Liver Injury via Modulating Myeloid Cell Chemokine (C-C Motif) Ligand 2 Secretion through the NF-κB Signaling Pathway.
, (2025),
PMID: 39746506
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