Product Detail
Product NameMyc(Phospho-Ser373) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Myc only when phosphorylated at serine 373.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 373 derived from Human Myc.
Target NameMyc
ConjugateUnconjugated
Other Namesc-myc
Accession NoSwiss-Prot: P01106
NCBI Protein: NP_002458.2
Uniprot
P01106
Gene ID
4609;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from A431 cells untreated or treated with EGF using Myc(Phospho-Ser373) Antibody #11036.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc(Phospho-Ser373) Antibody #11036.
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.
If you have published an article using product 11036, please notify us so that we can cite your literature.
Yes
