Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > Elk1(Phospho-Thr417) Antibody

Elk1(Phospho-Thr417) Antibody#11038

Availability: Yes
Total Amount:$
Product Detail

Product NameElk1(Phospho-Thr417) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Elk1 only when phosphorylated at threonine 417.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of threonine 417(L-S-T(p)-P-V) derived from Human Elk1.

Target NameElk1

ConjugateUnconjugated

Other NamesELKV

Accession NoSwiss-Prot: P19419
NCBI Gene ID: 2002
NCBI mRNA: NM_001257168.1
NCBI Protein: NP_001244097.1

Uniprot P19419

Gene ID 2002;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details

Predicted MW: 47kd

Immunohistochemistry: 1:50~1:100

Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk-1 (phospho-Thr417) antibody (#11038).
Immunofluorescence staining of methanol-fixed HeLa cells using Elk-1 (phospho-Thr417) antibody (#11038, Red).

 Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.

Yang S.-H., Sharrocks A.D.EMBO J. 24:2161-2171(2005)

Zhang L., Yang S.H., Sharrocks A.D.Mol. Cell. Biol. 27:2861-2869(2007) 

If you have published an article using product 11038, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;