Product Detail
Product NameMEF2a(Phospho-Thr319) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsIF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of MEF2A only when phosphorylated at Thr319.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of Thr319 (V-T-T(p)-P-S) derived from Human MEF2A.
Target NameMEF2a
ConjugateUnconjugated
Other NamesMEF2; Serum response factor-like protein 1;
Accession NoSwiss-Prot: Q02078
NCBI Protein: NP_001124398.1
Uniprot
Q02078
Gene ID
4205;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 54kd
Immunofluorescence: 1:100~1:200
Immunofluorescence staining of methanol-fixed Hela cells using MEF2A(Phospho-Thr319) Antibody #11040.
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain
Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13
Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656
Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798
Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30
If you have published an article using product 11040, please notify us so that we can cite your literature.
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