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GATA1(Phospho-Ser142) Antibody#11041

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Product Detail

Product NameGATA1(Phospho-Ser142) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of GATA1 only when phosphorylated at serine 142.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 142 (R-L-S(p)-P-D) derived from Human GATA1.

Target NameGATA1

ConjugateUnconjugated

Other NamesGAT1; GATA1; GF-1; NF-E1;

Accession NoSwiss-Prot: P15976
NCBI Protein: NP_002040.1

Uniprot P15976

Gene ID 2623;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HL60 cells untreated or treated with PMA using GATA1(Phospho-Ser142) Antibody #11041.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1(Phospho-Ser142) Antibody #11041(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed K562 cells using GATA1 (Phospho-Ser142) Antibody #11041.

GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672.
Trainor C D, et al. (1990) Nature. 343: 92-96.
Nichols K E, et al. (2000) Nat Genet. 24: 266-270.

If you have published an article using product 11041, please notify us so that we can cite your literature.

et al,FAM122A Inhibits Erythroid Differentiation through GATA1. In Stem Cell Reports on 2020 Sep 8 by Jing Chen, Qiong Zhou, et al..PMID:32763160 , (2020),
PMID: 32763160

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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