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HNF4a(Phospho-Ser304) Antibody#11043

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Product Detail

Product NameHNF4a(Phospho-Ser304) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of HNF4a only when phosphorylated at serine 304.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 304 (L-R-S(p)-Q-V)derived from Human HNF4a.

Target NameHNF4a

ConjugateUnconjugated

Other NamesHNF4; TCF; MODY; HNF4A;

Accession NoSwiss-Prot: P41235
NCBI Protein: NP_000448.3

Uniprot P41235

Gene ID 3172;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 52kd
Western blotting: 1:1000
Western blot analysis of extracts from Hela cells untreated or treated with Anisomyclin using HNF4a(Phospho-Ser304) Antibody #11043.
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes,including hepatocyte nuclear factor 1 a, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Kritis A.A,et al.Gene 173:275-280(1996)
Drewes T,et al.Mol. Cell. Biol. 16:925-931(1996)
Yamagata K,et al.Nature 384:458-460(1996)
Tanaka T,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases

If you have published an article using product 11043, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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