Product Detail
Product NameSTAT3(Phospho-Ser727) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of STAT3 only when phosphorylated at serine 727.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-R) derived from Human STAT3.
Target NameSTAT3
ConjugateUnconjugated
Other NamesAPRF, HIES
Accession NoSwiss-Prot: P40763
NCBI Protein: NP_003141.2
Uniprot
P40763
Gene ID
6774;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 88kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela and HepG2 cells untreated or treated with PMA usingSTAT3(Phospho-Ser727) Antibody #11046.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3(Phospho-Ser727) Antibody #11046(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using STAT3(Phospho-Ser727) Antibody #11046.
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using STAT3 (Phospho-Ser727) Antibody #11046.
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Takeda K, et al. (1997) Proc Natl Acad Sci USA. 94: 3801-3804.
Catlett-Falcone R, et al. (1999) Immunity. 10: 105-115.
Garcia R, et al. (1998) J Biomed Sci. 5: 79-85.
Bromberg J F, et al. (1999) Cell. 98: 295-303.
If you have published an article using product 11046, please notify us so that we can cite your literature.
et al,Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells.
, (2012),
PMID:
22899336
et al,Canonical Notch Pathway Protects Hepatocytes from Ischemia/Reperfusion Injury in Mice by Repressing Reactive Oxygen Species Production Through JAK2/STAT3 Signaling.
, (2011),
PMID:
21633967
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861
, (2019),
PMID:
31594861
et al,Anti inflammatory and metabolic reprogramming effects of MENK produce antitumor response in CT26 tumor bearing mice.In J Leukoc Biol on 2020 Jan 29. by Tuo Y, Zhang Z, et al..PMID:31994797
, (2020),
PMID:
31994797
et al,Resistance to bleomycin-induced lung fibrosis in MMP-8 deficient mice is mediated by interleukin-10. In PLoS One on 2010 Oct 7 by Emilio Garc铆a-Prieto, Adri谩n
Gonz谩lez-L贸pez, et al..PMID: 20949050
, (2010),
PMID:
20949050
et al,Significance and relationship between Cripto-1 and p-STAT3 expression in gastric cancer and precancerous lesions. In World J Gastroenterol on 2010 Feb 7 by Jian-Guo Zhang, Jing Zhao, et al..PMID: 20128024
, (2010),
PMID:
20128024
et al,Neurotrophic effects of leukemia inhibitory factor on neural cells derived from human embryonic stem cells. In Stem Cells on 2012 Nov by Anirban Majumder, Saswati Banerjee, et al..PMID: 22899336
, (2012),
PMID:
22899336
More
et al,Induction of interleukin-10 is dependent on p38 mitogen-activated protein kinase pathway in macrophages infected with porcine reproductive and respiratory syndrome virus. In Virol J on 2012 Aug 21 by Jun Hou, Lianghai Wang, et al..PMID: 22909062
, (2012),
PMID: 22909062
et al,Protein Kinase CK2 Inhibition Down Modulates the NF-魏B and STAT3 Survival Pathways, Enhances the Cellular Proteotoxic Stress and Synergistically Boosts the Cytotoxic Effect of Bortezomib on Multiple Myeloma and Mantle Cell Lymphoma Cells. In PLoS One on 2013 Sep 27 by Sabrina Manni, Alessandra Brancalion, et al..PMID:24086494
, (2013),
PMID: 24086494
et al, Prognostic significance of serine-phosphorylated STAT3 expression in pT1-T2 oral tongue carcinoma.In Clin Exp Otorhinolaryngol On 2015 Sep by Elena Fasanaro , Claudia Staffieri et al..PMID:26330924
, (2015),
PMID: 26330924
et al,Blocking the bFGF/STAT3 interaction through specific signaling pathways induces apoptosis in glioblastoma cells.In J Neurooncol 2014 Oct by Jingchao Wu , Xuequan Feng et al..PMID: 25048528
, (2014),
PMID: 25048528
et al,Migration Suppression of Small Cell Lung Cancer by Polysaccharides from Nostoc commune Vaucher. In J Agric Food Chem on 2016 Aug 17 by Min Guo, Guo-Bin Ding,et al..PMID: 27465400
, (2016),
PMID: 27465400
et al,Pwp1 Is Required for the Differentiation Potential of Mouse Embryonic Stem Cells Through Regulating Stat3 Signaling.
In Stem Cells on 2015 Mar by Junwei Shen, Wenwen Jia,et al..PMID:25335925
, (2015),
PMID: 25335925
et al,Evaluation of the Prognostic Role of pSTAT3 Expression in Temporal Bone Squamous Cell Carcinoma
, (2013),
PMID: 24005169
et al,AHR Agonist ITE Boosted PD1 Antibody’s Effects by Inhibiting Myeloid-Derived Cells Suppressive Cells in an Orthotopic Mouse Glioma Model
, (2025),
PMID:
et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy.
, (2025),
PMID: 40122152
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