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ATF4(Phospho-Ser245) Antibody#11053

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Product Detail

Product NameATF4(Phospho-Ser245) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was purified by immunogen affinity chromatography.

ApplicationsWB, IHC, IF/IC

Species ReactivityHu,Ms,Rat

SpecificityThe antibody detects endogenous level of ATF4 only when phosphorylated at serine 245.

Immunogen TypePeptide-KLH

Immunogen DescKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S245 of human ATF4 protein. The exact sequence is proprietary.

Target NameATF4

ConjugateUnconjugated

Other NamesC/ATF; C/EBP-related ATF; CREB2

Accession NoSwiss-Prot: P18848
NCBI Protein: NP _001666.2

Uniprot P18848

Gene ID 468;

Concentration1.0mg/ml

FormulationLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:1000, IH 1:50 - 1:100, IF/IC 1:50 - 1:200

Western blot analysis of extracts from Hela cells untreated or treated with UV using ATF4(Phospho-Ser245) Antibody #11053.
Western Blot analysis of various cell lysis using Antibody diluted at 1:500.
Immunohistochemical analysis of ATF4 (Phospho-S245) staining in human breast cancer formalin fixed paraffin embedded tissue section.
Immunofluorescent analysis of ATF4 (Phospho-S245) staining in A549 cells.

If you have published an article using product 11053, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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