Product Detail
Product NameAkt(Phospho-Thr308) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt.
Target NameAkt
ConjugateUnconjugated
Other NamesC-AKT; PKB; PKB-alpha; RAC; RAC-PK-alpha
Accession NoSwiss-Prot: P31749
NCBI Protein: NP_001014431.1
Uniprot
P31749
Gene ID
207;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt(Phospho-Thr308) Antibody #11055.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody #11055 (left) or the same antibody preincubated with blocking peptide #51055 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt(Phospho-Thr308) Antibody #11055.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody #11055.
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody #11055.
Western blot analysis using Akt(Phospho-Thr308) Antibody #11055.
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Tremblay F, et al. (2005)Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005)J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005)Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
If you have published an article using product 11055, please notify us so that we can cite your literature.
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et al,T鈥恈adherin modulates endothelial barrier function. In J Cell Physiol on 2010 Apr by Jingyan Han, Mikhail A Kutuzov, et al..PMID: 20039275
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et al,Cadmium-induced ER Stress and Inflammation Are Mediated Through C/EBP-DDIT3 Signaling in Human Bronchial Epithelial Cells.In Exp Mol Med on 2017 Sep by Jeeyoung Kim , Haengseok Song,et al..PMID: 28860664
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et al,Choline Prevents Fetal Overgrowth and Normalizes Placental Fatty Acid and Glucose Metabolism in a Mouse Model of Maternal Obesity.In J Nutr Biochem on 2017 Nov by Juha Nam , Esther Greenwald,et al..PMID: 28915389
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et al,H2O2 treatment or serum deprivation induces autophagy and apoptosis in naked mole-rat skin fibroblasts by inhibiting the PI3K/Akt signaling pathway.In Oncotarget on 2016 Dec 20 by Shanmin Zhao, Li Li et al..PMID:27863375
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et al,The combined anticancer of peanut skin procyanidins and resveratrol to CACO‐2 colorectal cancer cellsInFood Sci NutrOn2023 Aug 3byNa Wang,Enguang Gao et al..PMID: 37831732
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et al,AMOTL2 inhibits JUN Thr239 dephosphorylation by binding PPP2R2A to suppress the proliferation in non-small cell lung cancer cells
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