Product Detail
Product NameFAK(Phospho-Tyr861) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of FAK only when phosphorylated at tyrosine 861.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around phosphorylation site of tyrosine 861 (H-I-Y(p)-Q-P) derived from Human FAK.
Target NameFAK
ConjugateUnconjugated
Other NamesFADK 1; FAK1; PTK2
Accession NoSwiss-Prot: Q05397
NCBI Protein: NP _005598.3
Uniprot
Q05397
Gene ID
5747;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using FAK(Phospho-Tyr861) Antibody #11059.
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
If you have published an article using product 11059, please notify us so that we can cite your literature.
et al,Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration.
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PMID:
18987023
et al,Abnormal cleavage of APP impairs its functions in cell adhesion and migration
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PMID:
19056463
et al,Zoledronate inhibits the proliferation,adhesion and migration of vascular smooth muscle cells.
, (2008),
PMID:
19000670
et al,Targeting Lysophosphatidic Acid Signaling Retards Culture-Associated Senescence of Human Marrow Stromal Cells.
, (2012),
PMID:
22359668
et al,Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells.In PLoS One on 2012
by Kanehira M, Kikuchi T, et al..PMID:22359668
, (2012),
PMID:
22359668
et al,Abnormal cleavage of APP impairs its functions in cell adhesion and migration.In Neurosci Lett on 2009 Feb 6 by Baiyang Sheng, Bo Song,et al..PMID:19056463
, (2009),
PMID:
19056463
et al,Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration. In J Biomater Appl on 2009 Sep by
Zhenhuan Zheng, Yujun Wei, et al..PMID: 18987023
, (2009),
PMID:
18987023
More
et al,Zoledronate inhibits the proliferation, adhesion and migration of vascular smooth muscle cells. In Eur J Pharmacol 2009 Jan 5 by Liang Wu, Lei Zhu, et al..PMID:
19000670
, (2009),
PMID: 19000670
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer
, (2021),
PMID: 34830928
Yes
