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BCL-2(Phospho-Ser70) Antibody#11065

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Product Detail

Product NameBCL-2(Phospho-Ser70) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of BCL-2 only when phosphorylated at serine70.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 70(R-T-S(p)-P-L) derived from Human BCL-2.

Target NameBCL-2

ConjugateUnconjugated

Other NamesBcl-2; PPP1R50

Accession NoSwiss-Prot: P10415; NCBI Gene ID: 596; NCBI mRNA: NM_000633.2; NCBI Protein: NP_000624.2

Uniprot P10415

Gene ID 596;

Sdspage MW26KD

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details
 Predicted MW: 26kd
Immunohistochemistry: 1:50~1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using BCL-2 (phospho-Ser70) antibody (#11065).

 Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

If you have published an article using product 11065, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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