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BAD(Phospho-Ser112) Antibody#11067

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Product Detail

Product NameBAD(Phospho-Ser112) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of BAD only when phosphorylated at serine 112.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 112 (H-S-S(p)-Y-P) derived from Mouse BAD.

Target NameBAD

ConjugateUnconjugated

Other NamesBbc2

Accession NoSwiss-Prot: Q61337
NCBI Protein: NP_031548.1

Uniprot Q61337

Gene ID 12015;

Sdspage MW23kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody #11067
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser112) Antibody #11067(left) or the same antibody preincubated with blocking peptide(right).

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214.
Rice PL, et al. (2003). Cancer Res.63 (3): 616-620.
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.

If you have published an article using product 11067, please notify us so that we can cite your literature.

et al,Negative regulation of NaF-induced apoptosis by Bad鈥揅AII complex.In Toxicology on 2011 Sep 5 by S Otsuki, K Sugiyama,et al..PMID:21708216 , (2011),
PMID: 21708216

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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