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BAD(Phospho-Ser136) Antibody#11068

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Product Detail

Product NameBAD(Phospho-Ser136) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of BAD only when phosphorylated at serine136.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine136 (S-R-S(p)-A-P) derived from Mouse BAD.

Target NameBAD

ConjugateUnconjugated

Other NamesBbc2

Accession NoSwiss-Prot: Q61337
NCBI Protein: NP_031548.1

Uniprot Q61337

Gene ID 12015;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29 cells untreated or treated with UV using BAD(Phospho-Ser136) Antibody #11068.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Phospho-Ser136) Antibody #11068(left) or the same antibody preincubated with blocking peptide(right).

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.
Lee YI, et al. (2001). J Biol Chem.276 (20): 16969-16977.
Maiti D, et al. (2001). J Biol Chem.276 (1): 329-333.

If you have published an article using product 11068, please notify us so that we can cite your literature.

et al,Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. , (2007),
PMID: 17601517
et al,Negative regulation of NaF-induced apoptosis by Bad篓CCAII complex. , (2011),
PMID: 21708216
et al,miR-137 and miR-197 induce apoptosis and suppress tumorigenicity by targeting MCL-1 in multiple myeloma.In Clin Cancer Res on 2015 May 15 by Yijun Yang , Fei Li et al..PMID:25724519 , (2015),
PMID: 25724519

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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