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BAD(Phospho-Ser155) Antibody#11069

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Product Detail

Product NameBAD(Phospho-Ser155) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of BAD only when phosphorylated at serine 155.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 155(R-M-S(p)-D-E) derived from Human BAD.

Target NameBAD

ConjugateUnconjugated

Other NamesBbc2; AI325008

Accession NoSwiss-Prot: Q61337
NCBI Gene ID: 12015
NCBI mRNA: NM_007522.2
NCBI Protein: NP_031548.1

Uniprot Q61337

Gene ID 12015;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C

Application Details

Predicted MW: 23kd

Western blotting: 1:500

Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells using BAD (phospho-Ser155) antibody (#11069).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho- Ser155) antibody (#11069).

 Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Schurmann A., Mooney A.F., Sanders L.C., Sells M.A., Wang H.G., Reed J.C., Bokoch G.M.Mol. Cell. Biol. 20:453-461(2000)
Jakobi R., Moertl E., Koeppel M.A.J. Biol. Chem. 276:16624-16634(2001)        

If you have published an article using product 11069, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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