Product NameHER2(Phospho-Tyr877) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of HER2 only when phosphorylated at tyrosine 877.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from Human HER2.

Target NameHER2

ConjugateUnconjugated

Other NamesC-erbB-2; ErbB2;

Accession NoSwiss-Prot: P04626
NCBI Protein: NP_001005862.1

Uniprot P04626

Gene ID 2064;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200