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ICAM-1(Phospho-Tyr512) Antibody#11083

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Product Detail

Product NameICAM-1(Phospho-Tyr512) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous level of ICAM-1 only when phosphorylated at tyrosine 512.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1.

Target NameICAM-1

ConjugateUnconjugated

Other NamesICA1; ICAM1;

Accession NoSwiss-Prot: P05362
NCBI Protein: NP_000192.2

Uniprot P05362

Gene ID 3383;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 89 92kd
Western blotting: 1:500~1:1000
 

Western blot analysis of extracts from HUVEC cells using ICAM-1(Phospho-Tyr512) Antibody #11083(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody #11083.

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin a-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Greenwood J, et al. (2003) J Immunol; 171(4):2099-2108.
Zhou Z, et al. (2005) Eur J Pharmacol; 513(1-2):1-8.
Chen YH, et al. (2001) J Cell Biochem; 82(3):512-521
 

If you have published an article using product 11083, please notify us so that we can cite your literature.

et al,Impaired CD200鈥揅D200R-mediated microglia silencing enhances midbrain dopaminergic neurodegeneration: Roles of aging, superoxide, NADPH oxidase, and p38 MAPK. In Free Radic Biol Med on 2011 May 1 by Xi-Jin Wang, Shi Zhang, et al..PMID: 21295135 , (2011),
PMID: 21295135

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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