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VEGFR2(Phospho-Tyr1175) Antibody#11084

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Product Detail

Product NameVEGFR2(Phospho-Tyr1175) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsIHC IF

Species ReactivityHu Ms Rt

SpecificityThe Antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1175.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1175 (K-D-Y(p)-I-V) derived from Human VEGFR2.

Target NameVEGFR2

ConjugateUnconjugated

Other NamesFLK1; KDR; VGFR2; VGR2; kinase insert domain receptor

Accession NoSwiss-Prot: P35968
NCBI Protein: NP_002244.1

Uniprot P35968

Gene ID 3791;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 230kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using VEGFR2(Phospho-Tyr1175) Antibody #11084(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF cells using VEGFR2(Phospho-Tyr1175) Antibody #11084.
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions

Lamalice L, et al. (2004). Oncogene.23(2): 434-445.
Takahashi T, et al. (2001). EMBO J .20(11): 2768-2778.

If you have published an article using product 11084, please notify us so that we can cite your literature.

et al,Endogenous acetylcholine regulates neuronal and astrocytic vascular endothelial growth factor expression levels via different acetylcholine receptor mechanisms.In Neurochem Int. On 2018 Sep by Kimura K, Matsumoto K et al..PMID: 29705288 , (2018),
PMID: 29705288

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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