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IGF-1R(Phospho-Tyr1165/Tyr1166) Antibody#11088

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Product Detail

Product NameIGF-1R(Phospho-Tyr1165/Tyr1166) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB, IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of IGF-1R only when phosphorylated at tyrosine 1165/tyrosine1166.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 1165/tyrosine 1166 (T-D-Y(p)-Y(p)-R-K) derived from Human IGF-1R .

Target NameIGF-1R

ConjugateUnconjugated

Other Names Insulin-like growth factor I receptor; CD221; IGF1R; kinase IGF1R;

Accession NoSwiss-Prot: P08069
NCBI Protein: NP_000866.1

Uniprot P08069

Gene ID 3480;

Concentration1.0mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

WB 1:500 - 1:1000, IHC 1:50 - 1:100

Western blot analysis of IGF1 Receptor(Phospho-Y1165/Y1166) expression in MCF7 (A),MCF7-insulin-15min (B), A2780 (C) whole cell lysates.(Predicted band size: 154 kD; Observed band size: 95; 200 kD)
Immunohistochemical analysis of IGF1 Receptor(Phospho-Y1165/Y1166) staining in human colorectal cancerformalin fixed paraffin embedded tissue section.

This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.

Li S, et al. (1994) J Biol Chem; 269(51): 32558-64.

If you have published an article using product 11088, please notify us so that we can cite your literature.

et al,Tanshinone IIA Attenuates Insulin Like Growth Factor 1 -Induced Cell Proliferation in PC12 Cells through the PI3K/Akt and MEK/ERK Pathways.In Int J Mol Sci. On 2018 Sep 12 by Wang H, Su X et al..PMID: 30213025 , (2018),
PMID: 30213025
et al,PristimerIn attenuates cell proliferation of uveal melanoma cells by InhibitIng InsulIn-like growth factor-1 receptor and its downstream pathways. In J Cell Mol Med on 2019 Nov by Xie X, Xie S, et al..PMID:31508890 , (2019),
PMID: 31508890
et al,Glutamate Attenuates IGF-1 Receptor Tyrosine Phosphorylation in Mouse Brain: Possible Significance in Ischemic Brain Damage.In Neurosci Res on 2012 Dec by Chengming Sun, Qian Meng,et al..PMID:23085572 , (2012),
PMID: 23085572
et al,H 2 O 2 Attenuates IGF-1R Tyrosine Phosphorylation and Its Survival Signaling Properties in Neuronal Cells via NR2B Containing NMDA Receptor.In Oncotarget on 2017 Jun 27 by Zhiwen Zeng, Dejun Wang,et al..PMID: 29029433 , (2017),
PMID: 29029433

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;