Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Phospho Antibodies > Paxillin(Phospho-Tyr118) Antibody

Paxillin(Phospho-Tyr118) Antibody#11089

Availability: Yes
Total Amount:$
Product Detail

Product NamePaxillin(Phospho-Tyr118) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of tyrosine 118 (H-V-Y(p)-S-F) derived from Human Paxillin.

Target NamePaxillin

ConjugateUnconjugated

Other NamesPAXI; PXN;

Accession NoSwiss-Prot: P49023
NCBI Protein: NP_001074324.1

Uniprot P49023

Gene ID 5829;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3 cells untreated or treated with serum using Paxillin(Phospho-Tyr118) Antibody #11089.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin(Phospho-Tyr118) Antibody #11089(left) or the same antibody preincubated with blocking peptide(right).
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Tao WA, et al.(2005)Nat Methods.2(8): 591-598.
Zhang Y, et al.(2005) Mol Cell Proteomics.4(9): 1240-1250.
Sanders MA, et al.(2005)J Biol Chem.280(25): 23516-23522.
Rush J, et al.(2004) Nat Biotechnol. 23(1): 94-101.

If you have published an article using product 11089, please notify us so that we can cite your literature.

et al,Discovery of a novel inhibitor of the protein tyrosine phosphatase Shp2.In Sci Rep on 2015 Dec 2 by Chuan Chen , Mengmeng Cao et al..PMID: 26626996 , (2015),
PMID: 26626996

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;