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PKD/PKCm(Phospho-Ser910) Antibody#11096

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Product Detail

Product NamePKD/PKCm(Phospho-Ser910) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

ApplicationsWB IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of PKD/PKCm only when phosphorylated at serine 910.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around phosphorylation site of serine 910 (R-V-S(p)-I-L) derived from Human PKD/PKCm.

Target NamePKD/PKCm

ConjugateUnconjugated

Other NamesKPCD1; PKC-mu; PKCM; PKD; PRKCM

Accession NoSwiss-Prot: Q15139
NCBI Protein: NP_002733.2

Uniprot Q15139

Gene ID 5587;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 115kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCm(Phospho-Ser910) Antibody #11096.
Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser910) Antibody #11096.
Converts transient diacylglycerol. (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.

Matthews SA, et al. J Biol Chem 1999 Sep; 274(37): 26543-26549
Brandlin I, et al. J Biol Chem 2002 Feb; 277(8): 6490-6496
Storz P, et al. Mol Pharmacol 2004 Oct; 66(4): 870-879

If you have published an article using product 11096, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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